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Sangon Biotech single strand sgrna oligonucleotides
Single Strand Sgrna Oligonucleotides, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/single+strand+sgrna+oligonucleotides/base+dna+mismatched+single/pm42161958-259-0-5
Average 86 stars, based on 1 article reviews
single strand sgrna oligonucleotides - by Bioz Stars, 2026-10
86/100 stars

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Synthesized:

Article Title: Acetylated mitochondrial MDH2 regulates CTR2 transcription to induce cuproptosis during Escherichia coli infection.
Article Snippet: Gene knockout vector preparation: CRISPR/Cas9 vectors were constructed using the pSpCas9(BB)-2A-GFP (PX458) backbone (Addgene #48138) 60. sgRNAs targeting CTR1 (gRNA1: 5′-GAGTGTGAGGCTGAAGTGGT-3′), CTR2 (gRNA1: 5′- TTTCATCTTCTCAGATACAG-3′, gRNA2: 5′-GCAGGAGCACCAACACCGAA-3′), MDH2 (gRNA: 5′-GGTGTTGAACAGGTCGTCCC-3′), and TLR4 (gRNA: 5′- TCTCTAGAGAACTTCCCCAT-3′) were designed using CRISPOR (https://crispor.gi.ucsc.edu/crispor.py). .. Single-strand sgRNA oligonucleotides synthesized by Sangon Biotech (Shanghai, China) were phosphorylated, annealed, and ligated into BbsI-digested vectors using T4 ligase. ..



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